Theory of Photosynthesis
Photosynthesis is the physico-chemical process by which green plants use light energy to make organic compounds from carbon dioxide and water, releasing oxygen. This page covers what simple leaf experiments show, the classic experiments of Priestley, Ingenhousz, Sachs, Engelmann and van Niel, the overall equation of photosynthesis and the chloroplast as its site, following the NCERT Class 11 chapter Photosynthesis in Higher Plants. NEET often asks scientist and discovery pairs, the source of the released oxygen and the division of labour inside the chloroplast.
- ★ Must learn Photosynthesis: light energy drives the synthesis of organic compounds; green plants are autotrophs.
- It is the primary source of all food on earth and releases into the atmosphere.
- Starch tests on leaves show that chlorophyll, light and are all needed.
- ★ Must learn Priestley (1770): plants restore the air spoiled by a burning candle or a breathing mouse; he discovered oxygen in 1774.
- Ingenhousz: sunlight is essential, and only the green parts of a plant release oxygen.
- Sachs (about 1854): glucose is made in green parts and stored as starch; later he showed that chlorophyll lies in chloroplasts.
- ★ Must learn Engelmann: Cladophora, aerobic bacteria and a prism gave the first action spectrum (blue and red light).
- ★ Must learn van Niel: a hydrogen donor reduces ; in green plants is oxidised, so the released comes from water.
- Overall equation: + → + + , in light.
- ★ Must learn Chloroplast membranes (grana, stroma lamellae) carry out the light reactions and make ATP and NADPH; the stroma carries out the dark (carbon) reactions.
- Dark reactions are not light-independent: they need the ATP and NADPH made in light.
1. What Photosynthesis Is
- Biology studies life at two levels: the organism and above (ecology), and the cell and molecule (physiology and biochemistry).
- Photosynthesis is a physiological process. It is described in molecular terms, within the activities of cells and of the whole plant.
- All animals, including human beings, depend on plants for their food.
- Green plants make, or synthesise, their own food by photosynthesis. Hence they are called autotrophs.
- Autotrophs: organisms that synthesise their own food. In this sense, autotrophic nutrition is found only in plants.
- Heterotrophs: organisms that depend on green plants for their food.
- All living forms on earth ultimately depend on sunlight for energy.
- The use of sunlight by photosynthesising plants is therefore the basis of life on earth.
★ Very important Photosynthesis: a physico-chemical process in which green plants use light energy to drive the synthesis of organic compounds.
Why photosynthesis is important
- ★ Exam imp It is the primary source of all food on earth.
- It releases oxygen into the atmosphere, which organisms need to breathe.
Photosynthesis gives the living world two things: Food and Oxygen. Remember "F and O" for its two reasons of importance.
2. What Simple Experiments Show
- Simple school experiments show that chlorophyll (the green pigment of the leaf), light and are required for photosynthesis.
- The leaves are tested for starch, because starch forms only where photosynthesis has taken place.
| Experiment | Set-up | Result | Conclusion |
|---|---|---|---|
| Variegated leaf | A leaf with green and non-green patches is kept in light | Only the green parts test positive for starch | Chlorophyll is needed |
| Partly covered leaf | Part of a leaf is covered with black paper and kept in light | Only the uncovered (lit) part forms starch | Light is needed |
| Half-leaf in a test tube | Part of a leaf is sealed in a tube with -soaked cotton, which absorbs ; the other part is in air; all kept in light | Exposed part: starch present. Part in the tube: no starch | is needed |
3. Early Experiments
A series of simple experiments, done over about two centuries, slowly built our understanding of photosynthesis.
3.1 Joseph Priestley
- Joseph Priestley (1733-1804) performed a series of experiments in 1770.
- They showed the essential role of air in the growth of green plants.
- ★ Exam imp Priestley also discovered oxygen, in 1774.
- A candle burning in a closed space, a bell jar, soon goes out.
- A mouse kept in a closed bell jar soon suffocates.
- Priestley concluded that a burning candle or a breathing animal somehow damages the air.
- He then placed a mint plant in the same bell jar. The mouse stayed alive and the candle kept burning.
- Hypothesis: plants restore to the air whatever breathing animals and burning candles remove.
To test whether the air was restored, the candle had to be rekindled after a few days. This had to be done without opening the jar, for example by focusing sunlight on the wick with a lens.
3.2 Jan Ingenhousz
- Jan Ingenhousz (1730-1799) used a set-up like Priestley's, placed once in the dark and once in sunlight.
- ★ Exam imp He showed that sunlight is essential for the plant process that purifies air fouled by burning candles or breathing animals.
- With an aquatic plant, he saw small bubbles form around the green parts in bright sunlight, but not in the dark.
- He later identified these bubbles as oxygen.
- Hence he showed that only the green parts of a plant can release oxygen.
3.3 Julius von Sachs
- In about 1854, Julius von Sachs gave evidence that glucose is produced when plants grow.
- ★ Exam imp Glucose is usually stored as starch.
- His later studies showed that the green substance (chlorophyll) lies in special bodies within plant cells, later called chloroplasts.
- He found that glucose is made in the green parts of the plant.
3.4 T.W. Engelmann
- T.W. Engelmann (1843-1909) split light into its spectral components using a prism.
- He illuminated a green alga, Cladophora, kept in a suspension of aerobic bacteria.
- The aerobic bacteria detected the sites of evolution, because they gather where oxygen is released.
- ★ Exam imp The bacteria accumulated mainly in the regions of blue and red light.
- This gave the first action spectrum of photosynthesis. It roughly resembles the absorption spectra of chlorophyll a and b.
★ Very important Engelmann's experiment = Cladophora (green alga) + aerobic bacteria + prism. It produced the first action spectrum, with peaks in the blue and red regions.
Match each first letter to the finding: Priestley: Plant restores air; Ingenhousz: Illumination needed; Sachs: Starch from glucose; Engelmann: Each colour of the spectrum tested.
Which plant did Priestley keep in the bell jar?
Which scientist showed that only the green parts of a plant release oxygen?
Which alga did Engelmann use, and what did the aerobic bacteria detect?
In what form is the glucose made in green parts usually stored?
4. The Equation of Photosynthesis
4.1 The empirical equation
- By the middle of the nineteenth century, the key features were known: plants use light energy to make carbohydrates from and water.
- The empirical equation for oxygen-evolving organisms was written as:
- represents a carbohydrate, for example glucose, a six-carbon sugar.
4.2 Cornelius van Niel
- Cornelius van Niel (1897-1985) was a microbiologist who studied purple and green bacteria.
- He showed that photosynthesis is essentially a light-dependent reaction.
- In it, hydrogen from a suitable oxidisable compound reduces to carbohydrates:
| Organism | Hydrogen donor () | Oxidation product (A) |
|---|---|---|
| Green plants | ||
| Purple and green sulphur bacteria | Sulphur or sulphate (no released) |
- Some organisms, therefore, do not release during photosynthesis.
- ★ Exam imp van Niel inferred that the evolved by green plants comes from water, not from carbon dioxide.
- This was later proved by radioisotopic techniques.
The oxygen released in photosynthesis comes from water. van Niel inferred it from sulphur bacteria, and radioisotope techniques later proved it. A statement saying the comes from is false.
4.3 The correct overall equation
- represents glucose.
- ★ Exam imp This equation describes a multistep process, not a single reaction.
★ Very important Why 12 ? All 6 (12 oxygen atoms) come from water, so 12 water molecules must be split. The oxygen atoms of end up in glucose and in the 6 formed as a product.
Order of the scientists: "Plants In Sunlight Evolve Vital oxygen" = Priestley, Ingenhousz, Sachs, Engelmann, van Niel.
5. Where Photosynthesis Takes Place
- Photosynthesis takes place mainly in the green leaves, and also in other green parts of the plant.
- Leaves take in from the atmosphere through their stomata.
- ★ Exam imp The mesophyll cells of leaves have a large number of chloroplasts.
- Chloroplasts usually align themselves along the walls of the mesophyll cells, so that they get the optimum quantity of incident light.
5.1 Structure of the chloroplast
- Within the chloroplast is a membranous system made of grana and stroma lamellae, lying in a matrix called the stroma.
- Parts labelled in an electron micrograph section: outer membrane, inner membrane, stromal lamella, grana, stroma, ribosomes, starch granule and lipid droplet.
Name the double boundary of the chloroplast.
What are the stacks of discs, and what joins them?
Name the matrix and three things seen in it.
5.2 Division of labour in the chloroplast
| Part | What happens | Name of the reactions |
|---|---|---|
| Membrane system (grana and stroma lamellae) | Traps light energy; synthesises ATP and NADPH | Light reactions (photochemical reactions): directly light driven |
| Stroma | Enzymatic reactions synthesise sugar, which forms starch | Dark reactions (carbon reactions): depend on ATP and NADPH from the light reactions |
★ Very important The name dark reactions is only a convention. They do not occur in darkness, and they are not light-independent, because they need the ATP and NADPH made in light.
- Site: thylakoid membranes (grana, stroma lamellae)
- Directly driven by light
- Products: ATP and NADPH (and )
- Site: stroma
- Not directly driven by light
- Use ATP and NADPH to make sugar and starch
Name a plant part other than the leaf that can photosynthesise.
Why do chloroplasts line the walls of mesophyll cells?
Which part of the chloroplast makes ATP and NADPH, and which makes sugar?
"Membranes Make energy, Stroma Synthesises sugar": M for membranes and ATP/NADPH, S for stroma and sugar.
6. Exam Essentials
Pairs to Match
| List I | List II |
|---|---|
| Joseph Priestley (1770) | Plants restore air spoiled by candles and animals (mint plant) |
| Joseph Priestley (1774) | Discovery of oxygen |
| Jan Ingenhousz | Sunlight is essential; oxygen bubbles only from green parts |
| Julius von Sachs (about 1854) | Glucose made in green parts, stored as starch; later, chlorophyll in chloroplasts |
| T.W. Engelmann | First action spectrum (Cladophora, aerobic bacteria, prism) |
| Cornelius van Niel | Hydrogen donor reduces ; comes from water |
| Radioisotope techniques | Proved that the released comes from water |
| -soaked cotton | Absorbs |
| Variegated leaf | Starch only in the green parts |
| Aerobic bacteria | Detect sites of evolution |
| Purple and green sulphur bacteria | as hydrogen donor; sulphur or sulphate formed |
| Grana and stroma lamellae | Light reactions; ATP and NADPH |
| Stroma | Dark (carbon) reactions; sugar and starch |
Exceptions
- Photosynthesis is not only in leaves; other green parts also photosynthesise.
- Purple and green sulphur bacteria photosynthesise but do not release .
- The released comes from water, not from .
- Dark reactions do not take place in darkness and are not independent of light.
- Photosynthesis is not a single reaction but a multistep process.
- Water is both used (12 ) and formed (6 ) in the overall equation.
Numbers to Remember
- Priestley: 1733-1804; experiments in 1770; discovered oxygen in 1774.
- Ingenhousz: 1730-1799.
- Sachs: evidence for glucose in about 1854.
- Engelmann: 1843-1909. van Niel: 1897-1985.
- Overall equation: 6 + 12 → 1 glucose + 6 + 6 .
- Glucose is a six-carbon sugar; leaf colour comes from four pigments (next page).
7. Quick Revision
- Photosynthesis: light energy drives the synthesis of organic compounds in green plants.
- Green plants are autotrophs; organisms that depend on them are heterotrophs.
- Two reasons it matters: primary source of all food, and release of oxygen.
- Chlorophyll, light and are needed (variegated leaf, black paper, half-leaf tests).
- Priestley (1770): mint plant restores air; discovered oxygen in 1774.
- Ingenhousz: sunlight needed; only green parts release oxygen bubbles.
- Sachs (about 1854): glucose made in green parts, stored as starch; later, chlorophyll in chloroplasts.
- Engelmann: Cladophora + aerobic bacteria + prism = first action spectrum (blue and red).
- van Niel: + → 2A + + ; sulphur bacteria use .
- released comes from water, proved by radioisotope techniques.
- + → + + : a multistep process.
- Mesophyll cells have many chloroplasts aligned along their walls.
- Membranes: light reactions, ATP and NADPH. Stroma: dark (carbon) reactions, sugar and starch.
- Dark reactions are named by convention; they are not light-independent.
8. Solved Examples
List I: A. Priestley; B. Ingenhousz; C. Engelmann; D. van Niel
List II: I. comes from water; II. First action spectrum; III. Bubbles only from green parts in sunlight; IV. Mint plant restores air
Choose the correct answer.
(A) A-IV, B-III, C-II, D-I
(B) A-III, B-IV, C-II, D-I
(C) A-IV, B-II, C-III, D-I
(D) A-I, B-III, C-II, D-IV
Answer: (A). Priestley used a mint plant (IV); Ingenhousz saw oxygen bubbles only around green parts in light (III); Engelmann obtained the first action spectrum (II); van Niel inferred that oxygen comes from water (I).
A. Priestley discovered oxygen in 1774.
B. Sachs showed that glucose is usually stored as starch.
C. Engelmann used a moss with anaerobic bacteria.
D. van Niel studied purple and green bacteria.
E. Ingenhousz showed that sunlight is not needed to purify air.
Choose the correct answer.
(A) A, B and D only
(B) A, C and E only
(C) B, C and D only
(D) A, B, D and E only
Answer: (A). C is false: Engelmann used the green alga Cladophora with aerobic bacteria. E is false: Ingenhousz showed that sunlight is essential. A, B and D are true.
A. Engelmann
B. Priestley
C. van Niel
D. Sachs
E. Ingenhousz
Choose the correct order.
(A) B, E, D, A, C
(B) E, B, D, A, C
(C) B, E, A, D, C
(D) B, D, E, A, C
Answer: (A). Priestley worked in 1770. Ingenhousz repeated Priestley's set-up, so his work came next. Sachs followed in about 1854, Engelmann's prism experiment came later in the nineteenth century, and van Niel worked in the twentieth century. The phrase "Plants In Sunlight Evolve Vital oxygen" gives this order.
(A) Light is needed for photosynthesis
(B) is needed for photosynthesis
(C) Chlorophyll is needed for photosynthesis
(D) destroys starch
Answer: (B). absorbs . Light, water and chlorophyll are the same for both halves, so the missing starch is due only to the missing .
(A) They take place in the stroma
(B) They use ATP and NADPH
(C) They take place only in darkness
(D) They are also called carbon reactions
Answer: (C). The name is only a convention. The dark reactions run in light too, and they depend on the products of the light reactions.
(A) 6 split, 12 formed
(B) 12 split, 6 formed
(C) 6 split, 6 formed
(D) 12 split, 12 formed
Answer: (B). Six molecules contain 12 oxygen atoms, all from water, so 12 are split. Six appear again as a product.
9. Practice Questions
- Match List I with List II.
List I: A. Grana; B. Stroma; C. Mesophyll cells; D. Stomata
List II: I. Entry of into the leaf; II. Light reactions; ATP and NADPH; III. Dark reactions; sugar and starch; IV. Many chloroplasts in the leaf
Choose the correct answer.
(A) A-II, B-III, C-IV, D-I
(B) A-III, B-II, C-IV, D-I
(C) A-II, B-III, C-I, D-IV
(D) A-IV, B-III, C-II, D-IAnswer: (A). Grana: light reactions; stroma: dark reactions; mesophyll: many chloroplasts; stomata: entry of . - Read the statements about the chloroplast.
A. Chloroplasts align along the walls of mesophyll cells.
B. The membrane system traps light energy.
C. Starch granules and lipid droplets lie in the stroma.
D. Sugar is synthesised on the grana.
Choose the correct answer.
(A) A, B and C only
(B) A and D only
(C) B, C and D only
(D) A, B, C and DAnswer: (A). D is false: sugar is synthesised by enzymes in the stroma. - Statement I: In purple and green sulphur bacteria, is the hydrogen donor.
Statement II: These bacteria release oxygen during photosynthesis.
Choose the correct answer.
(A) Both Statement I and Statement II are correct
(B) Both Statement I and Statement II are incorrect
(C) Statement I is correct but Statement II is incorrect
(D) Statement I is incorrect but Statement II is correctAnswer: (C). They form sulphur or sulphate, not oxygen. - In Engelmann's experiment, the aerobic bacteria gathered mainly in the:
(A) Green and yellow light
(B) Blue and red light
(C) Violet and green light
(D) Orange and yellow lightAnswer: (B). Oxygen was released most in blue and red light, giving the first action spectrum. - Which experiment does NOT test the need for light?
(A) Leaf partly covered with black paper
(B) Ingenhousz's set-up kept in dark and in sunlight
(C) Half-leaf with -soaked cotton
(D) Aquatic plant bubbles in sunlight and in darkAnswer: (C). The experiment tests the need for ; both halves get light. - Arrange the steps of Priestley's experiment in order.
A. Mint plant placed in the bell jar
B. Candle goes out in a closed bell jar
C. Candle keeps burning and mouse stays alive
D. Conclusion that candles and animals damage the air
(A) B, D, A, C
(B) A, B, C, D
(C) B, A, D, C
(D) D, B, A, CAnswer: (A). Candle goes out, air is judged damaged, mint plant is added, and the candle and mouse then survive. - The oxygen released in photosynthesis was finally proved to come from water by:
(A) Paper chromatography
(B) Radioisotopic techniques
(C) Starch tests
(D) Use of a prismAnswer: (B). van Niel's inference was later confirmed with radioisotopes.
Common Mistakes to Avoid
- Writing that the released comes from . Correct: it comes from water.
- Calling the dark reactions light-independent or saying they occur at night. Correct: they need ATP and NADPH from light.
- Saying Engelmann used aerobic bacteria with a moss or with Spirogyra. Correct: a green alga, Cladophora.
- Mixing up the years: Priestley's experiments were in 1770 and his discovery of oxygen in 1774.
- Thinking photosynthesis occurs only in leaves. Other green parts photosynthesise too.
- Writing 6 on the left side of the equation. Correct: 12 used, 6 formed.
- Placing sugar synthesis on the grana. Correct: grana make ATP and NADPH; sugar forms in the stroma.
- Assuming every photosynthetic organism releases . Sulphur bacteria release sulphur or sulphate instead.
Frequently Asked Questions
What is photosynthesis?
Photosynthesis is the physico-chemical process in which green plants use light energy to make organic compounds, mainly glucose, from carbon dioxide and water. Oxygen is released as a by-product. It is the primary source of all food on earth and the source of atmospheric oxygen.
What did Priestley's experiment show?
In 1770 Priestley showed that a candle goes out and a mouse suffocates in a closed bell jar, but both survive when a mint plant is kept inside. He concluded that plants restore to the air whatever burning candles and breathing animals remove.
How did Engelmann obtain the first action spectrum?
Engelmann split light with a prism and shone it on the green alga Cladophora kept among aerobic bacteria. The bacteria gathered where oxygen was released, mainly in blue and red light. This first action spectrum roughly matches the absorption spectra of chlorophyll a and b.
Where does the oxygen released in photosynthesis come from?
It comes from water. Studying purple and green sulphur bacteria, which use and release sulphur instead of oxygen, van Niel inferred that green plants oxidise water to oxygen. Radioisotope techniques later proved this. It also explains why 12 water molecules appear in the overall equation.
Why are 12 water molecules used in the equation of photosynthesis?
The 6 oxygen molecules released contain 12 oxygen atoms, and all of them come from water. So 12 water molecules must be split. The oxygen of carbon dioxide goes into glucose and into the 6 water molecules formed as a product.
Why are dark reactions not truly dark?
Dark reactions are not driven directly by light, but they depend on the ATP and NADPH made in the light reactions. When light is removed they continue only briefly and then stop. So the name is a convention; they are better called carbon reactions.
What is the division of labour inside a chloroplast?
The membrane system of grana and stroma lamellae traps light energy and makes ATP and NADPH in the light reactions. The stroma uses them in enzymatic reactions to make sugar, which forms starch. These stroma reactions are the dark or carbon reactions.
Which early experiments of photosynthesis are important for NEET from NCERT?
Learn Priestley (1770, mint plant; oxygen 1774), Ingenhousz (sunlight and green parts), Sachs (1854, glucose and starch), Engelmann (Cladophora, first action spectrum) and van Niel (oxygen from water). NEET often pairs each scientist with his finding in match-the-list questions. Questions also test the organisms used, such as the mint plant and Cladophora.
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