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NEET 2023, Biology Q5: Recombinant DNA Technology

NEET2023Biology
Q.

Upon exposure to UV radiation, DNA stained with ethidium bromide will show:

  1. A

    Bright red colour

  2. B

    Bright blue colour

  3. C

    Bright yellow colour

  4. D

    Bright orange colour

Solution

Ethidium bromide intercalates between DNA base pairs and fluoresces brightly when excited by UV light, producing bright orange bands on an agarose gel. This is the routine visualisation step after electrophoresis in recombinant DNA work.

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