Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A) : Polymerase chain reaction is used in DNA amplification
Reason (R) : The ampicillin resistant gene is used as a selectable marker to check transformation.
In the light of the above statements, choose the correct answer from the options given below :
- A
Both (A) and (R) are correct and (R) is the correct explanation of (A)
- B
Both (A) and (R) are correct but (R) is not the correct explanation of (A)
- C
(A) is correct but (R) is not correct
- D
(A) is not correct but (R) is correct
Polymerase chain reaction (PCR) is a standard laboratory technique to amplify a specific DNA segment in vitro using thermostable DNA polymerase and primers. So Assertion (A) is correct.
Antibiotic resistance genes such as amp (ampicillin resistance) act as selectable markers in recombinant DNA technology: transformed host cells survive on the antibiotic-containing medium, while non-transformed cells die. So Reason (R) is also correct.
However, the two statements describe different procedures (PCR vs selection of transformants), so (R) does not explain (A). The answer is option (2).
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