Fundamentholfundamenthol
NEET2025Biology
Q.

Which of the following enzyme(s) are NOT essential for gene cloning?

A. Restriction enzymes

B. DNA ligase

C. DNA mutase

D. DNA recombinase

E. DNA polymerase

Choose the correct answer from the options given below:

  1. A

    B and C only

  2. B

    C and D only

  3. C

    A and B only

  4. D

    D and E only

Solution

Standard gene cloning relies on restriction enzymes (to cut DNA at specific sites), DNA ligase (to seal vector and insert), and DNA polymerase (to amplify or fill in DNA).

“DNA mutase” and “DNA recombinase” are not standard tools in routine gene cloning.

Not essential: C and D.

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