Fundamentholfundamenthol

NEET 2025, Biology Q27: Recombinant DNA Technology

NEET2025Biology
Q.

Which of the following enzyme(s) are NOT essential for gene cloning?

A. Restriction enzymes

B. DNA ligase

C. DNA mutase

D. DNA recombinase

E. DNA polymerase

Choose the correct answer from the options given below:

  1. A

    B and C only

  2. B

    C and D only

  3. C

    A and B only

  4. D

    D and E only

Solution

Standard gene cloning relies on restriction enzymes (to cut DNA at specific sites), DNA ligase (to seal vector and insert), and DNA polymerase (to amplify or fill in DNA).

“DNA mutase” and “DNA recombinase” are not standard tools in routine gene cloning.

Not essential: C and D.

Concept behind this question

Recombinant DNA TechnologyNotes, formulas and examples →

More previous year questions on Recombinant DNA Technology

Practice more Biology

Concept-wise practice with instant solutions on Fundamenthol.

Start practicing →