Arrange the following steps of DNA fingerprinting in a correct sequence.
A. Isolation of DNA and its digestion by restriction endonucleases.
B. Hybridisation using a labelled VNTR probe.
C. Transferring of separated DNA fragments to synthetic membranes.
D. Detection of hybridised DNA fragments by autoradiography.
E. Separation of DNA fragments by electrophoresis.
Choose the correct answer from the options given below :
- A
A, B, D, C, E
- B
A, D, B, E, C
- C
A, E, C, B, D
- D
A, E, B, C, D
The standard sequence of DNA fingerprinting is:
A. Isolation of DNA followed by digestion with restriction endonucleases.
E. Separation of the resulting DNA fragments by gel electrophoresis according to size.
C. Transfer of the separated fragments to a synthetic membrane (nylon or nitrocellulose).
B. Hybridisation of the membrane-bound fragments with a labelled VNTR probe.
D. Visualisation of the hybridised bands by autoradiography using X-rays.
So the order is A → E → C → B → D.
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