Fundamentholfundamenthol
NEET2022Biology
Q.

In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme ?

  1. A

    5' G A T A C T 3' ; 3' C T A T G A 5'

  2. B

    5' G A A T T C 3' ; 3' C T T A A G 5'

  3. C

    5' C T C A G T 3' ; 3' G A G T C A 5'

  4. D

    5' G T A T T C 3' ; 3' C A T A A G 5'

Solution

A true palindromic DNA sequence reads the same on both strands in the 5' to 3' direction. The sequence 5' G A A T T C 3' / 3' C T T A A G 5' satisfies this and is the recognition site for the well-known restriction enzyme EcoRI.

EcoRI cuts between G and A on each strand, generating sticky (staggered) ends with overhanging AATT sequences. The other sequences listed are not palindromic in the strict double-stranded sense.

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