Given below are two statements :
Statement I : Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence.
Statements II : Restriction endonucleases cut the DNA strand a little away from the centre of the palindromic site.
In the light of the above statements, choose the most appropriate answer from the options given below :
- A
Both Statement I and Statement II are correct
- B
Both Statement I and Statement II are incorrect
- C
Statement I is correct but Statement II is incorrect
- D
Statement I is incorrect but Statement II is correct
Restriction endonucleases recognise specific palindromic nucleotide sequences in double-stranded DNA. Statement I is correct.
Most restriction enzymes do not cut at the exact centre of the palindrome but a little away from it, generating staggered cuts that leave single-stranded overhangs (sticky ends). Statement II is also correct.
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