Fundamentholfundamenthol
NEET2026Biology
Q.

Insertion of a foreign DNA at BamHI site in an E.coli cloning vector pBR322 results in the loss of antibiotic resistance towards:

  1. A

    Ampicillin and tetracycline

  2. B

    Ampicillin

  3. C

    Tetracycline

  4. D

    Gentamycin

Solution

The BamHI site in pBR322 lies within the tetracycline resistance gene. Inserting a foreign DNA at this site disrupts the gene, so the recombinant plasmid loses its tetracycline resistance.

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