NEET 2022, Biology Q87: Recombinant DNA Technology
Which of the following is not a desirable feature of a cloning vector ?
- A
Presence of origin of replication
- B
Presence of a marker gene
- C
Presence of single restriction enzyme site
- D
Presence of two or more recognition sites
An ideal cloning vector should possess: (i) an origin of replication (ori) so that it can replicate inside the host, (ii) one or more selectable marker genes (such as antibiotic resistance) to identify transformed cells, and (iii) a single recognition site for restriction enzymes within the marker, so the foreign DNA inserts at a known place.
If a vector has two or more recognition sites for the same enzyme, the vector itself would be fragmented during digestion, complicating the cloning process. Hence multiple sites are not desirable.
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