Which of the following statements are correct with respect to DNA separation, isolation and visualization?
A. The cutting of DNA is done by molecular scissors.
B. The DNA fragments separate according to their size in an agarose gel, upon electrophoresis.
C. The separated DNA fragments can be seen without staining when exposed to UV light.
D. The separated DNA fragments, when stained with ethidium bromide, can be seen in visible light.
Choose the correct answer from the options given below :
- A
B and D only
- B
A and B only
- C
B and C only
- D
A and D only
DNA is cut using restriction enzymes (molecular scissors) and the resulting fragments are separated according to size in an agarose gel by electrophoresis, where the gel provides a sieving effect.
The separated DNA fragments cannot be seen without staining; they need to be stained with ethidium bromide and then visualised under UV radiation (not visible light). Hence statements C and D are wrong, and statements A and B are correct.
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