Fundamentholfundamenthol
NEET2023Biology
Q.

Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.

A. Insertion of recombinant DNA into the host cell

B. Cutting of DNA at specific location by restriction enzyme

C. Isolation of desired DNA fragment

D. Amplification of gene of interest using PCR

Choose the correct answer from the options given below:

  1. A

    B, C, D, A

  2. B

    C, A, B, D

  3. C

    C, B, D, A

  4. D

    B, D, A, C

Solution

Logical order: first cut the source DNA with restriction enzymes (B), then isolate the desired fragment by electrophoresis (C), amplify it by PCR (D), and finally insert the amplified recombinant DNA into a suitable host cell (A).

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