The following diagram showing restriction sites in E. coli cloning vector pBR322. Find the role of 'X' and 'Y' genes:
- A
The gene 'X' is for protein involved in replication of Plasmid and 'Y' for resistance to antibiotics
- B
Gene 'X' is responsible for recognition sites and 'Y' is responsible for antibiotic resistance
- C
The gene 'X' is responsible for resistance to antibiotics and 'Y' for protein involved in the replication of Plasmid
- D
The gene 'X' is responsible for controlling the copy number of the linked DNA and 'Y' for protein involved in the replication of Plasmid
In pBR322, besides the antibiotic resistance genes, there are two important regions:
1. ori (Origin of Replication) – controls the copy number of the plasmid.
2. rop (Repressor of Primer) gene – encodes a protein involved in plasmid replication and copy number regulation.
In the given figure, X and Y are located in the lower part of the plasmid, away from the antibiotic resistance regions. Therefore, they do not represent the ampR or tetR genes.
X = ori (Origin of Replication)
Controls the copy number of the linked DNA.
Y = rop gene
Codes for a protein involved in plasmid replication.
Therefore,
Reason: X is responsible for controlling the copy number of the linked DNA, while Y codes for a protein involved in plasmid replication.
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